NGS Library Preparation | Stellvard Bioscience

NGS Library Preparation Services

u r next-generation sequencing library preparation services ensure high-efficiency translation of DNA and RNA samples into sequencing-ready libraries. Compatible with leading Illumina, PacBio, and Nanopore platforms, we handle ultra-low input samples, FFPE, single cells, and custom targets with maximum library complexity.

Sample Extraction & QC

Isolation of nucleic acids followed by controlled physical or enzymatic fragmentation to targeted insert sizes.

Adapter Ligation & Indexing

End-repair, A-tailing, and ligation of platform-specific adapters with unique dual indexes to enable multiplexed runs.

Library Amplification & QC

PCR enrichment and bead purification followed by high-sensitivity electrophoresis and qPCR library quantification.

Quality Control & Input Flexibility

High-quality library preparation is the key to successful sequencing. We specialize in handling challenging sample types, including highly degraded FFPE samples, low-input circulating cell-free DNA (cfDNA), and single cells. Our strict pre-library and post-library quality control checkpoints verify fragment sizes and sample purity, minimizing duplication rates and maximizing sequencing data output.